BIO 112IN - Bioscience Laboratory Fundamentals

1. Demonstrate laboratory techniques used in bioscience laboratories.
2. Demonstrate scientific literacy.

1. Practice proper laboratory safety.
2. Practice common laboratory procedures.
3. Demonstrate the ability to keep a legal scientific notebook compatible with industry standards.
4. Employ the correct mathematical rules of operation, and be able to apply these to the preparation of reagents and biological solutions.
5. Demonstrate techniques common to the bioscience laboratory.
6. Understand and demonstrate ability to perform biological separations.
7. Demonstrate ability to grow, transfer, and enumerate bacterial cells.
8. Research current topics and/or careers in bioscience.

1. Safety
   1. Risk assessment
   2. Responding to emergencies
   3. Working with chemicals
   4. Working with biological hazards
   5. Quality control/quality assurance
2. Laboratory Procedures
   1. Proper waste disposal
   2. Dishwashing
   3. Sterilization
3. Documentation
   1. Keeping a laboratory notebook
   2. Writing and following a Standard Operating Procedure
4. Laboratory Mathematics and Solution Preparation Measurements
   1. Metric System and calculations
   2. Percent solutions/dilutions
   3. Normal/Molar solutions
   4. Buffers
   5. Weight and volume measurement
   6. Significant figures in measurement
   7. pH measurement
5. Assays and Instrumentation
   1. Quantitative and qualitative assay of biological molecules
   2. Proper use of equipment
6. Biological Separation Methods
   1. Separation of materials using a centrifuge
   2. Agarose gel electrophoresis
   3. Other biological separation methods
7. Growing Cells
   1. Aseptic technique
   2. Culturing bacteria
   3. Enumerating colony-forming units
8. Research
   1. Utilize current research articles to become more aware of current biotechnology
   2. Explore opportunities for careers in the bioscience field