MLT 230IN - Immunohematology and Immunology

7 Contact Hours, 5 Credits
4 lecture periods 3 lab periods

Introduction to basic immunology related to methods utilized in the clinical laboratory. Includes blood collection, blood components, immunology and complement, principles of serological testing, genetics, blood group systems, antiglobulin testing, gel and solid phase testing, and identification of unexpected antibodies. Also includes neonatal and obstetrical transfusion practice, pre-transfusion compatibility testing, International Society of Blood Transfusion (ISBT) product labeling, adverse effects of blood transfusions, positive direct antiglobulin test (DAT), immune hemolysis, quality assurance, transplantation, and molecular testing.

Prerequisite(s): With a grade of B or better: BIO 205IN, and MAT 151 or assessment into MAT 189.
Information: Students must be admitted to the Medical Laboratory Technician program or obtain consent of instructor before enrolling in this course. MLT 230IN is equivalent to MLT 230 and MLT 230LB. Regular student attendance is required.

Course Learning Outcomes

Explain the donor selection process, product preparation, processing, International Society of Blood Transfusion (ISBT) labeling, testing, and use of blood components.
Distinguish the immune processes as they relate to immunohematology and the characteristics of antigens and antibodies of the ABO, Rh, and other blood group systems.
Obtain accurate results using a variety of methods on samples including ABO, Rh, DAT, IAT, crossmatch, phenotype (and control selection), donor selection; and test for adverse effects of transfusions.
Identify units, reagents and samples using standard labeling practices.
Improve critical thinking and organizational skills by active engagement in class activities and problem-solving discussions.

Outline:

Blood Collection
Blood and Blood Components
Immunology and Complement
Principles of Serological Testing in Immunohematology
Genetics
ABO and H Blood Group Systems
Rh Blood Group System
Other Blood Group Systems
Antiglobulin Testing
Gel and Solid Phase Testing
Identification of Unexpected Antibodies
Neonatal and Obstetrical Transfusion Practice
Pre-Transfusion Compatibility Testing
International Society of Blood Transfusion (ISBT) Product Labeling
Adverse Effects of Blood Transfusion
Investigation of the Positive Direct Antiglobulin Test (DAT) and Immune Hemolysis
Quality Assurance
Transplantation
Molecular Testing