MLT 250IN - Clinical Microbiology

1. Identify proper growth requirements for bacterial organisms, their clinical significance and susceptibility to antibiotics by interpreting smears, correlating specific microbiology test results with organisms and disease states, and interpreting susceptibility procedural results.
2. Employ basic techniques used in bacteriology, including proper operation of the compound microscope, aseptic handling of bacteriology specimens, preparation and staining bacteriological smears, selection of proper media, and performance of susceptibility procedures.
3. Improve critical thinking and organizational skills by attending class, completing assignments, and participating in problem-solving discussions.

1. Introduction
   1. Role of the clinical microbiologist
   2. Factors impacting the current and future operation of the microbiology laboratory
   3. Basic bacteriology concepts
      1. Taxonomy
      2. Morphology
      3. Genetics
      4. Metabolism
   4. Host microorganism interactions
2. Clinical Bacteriology Methodology
   1. Specimen collection, transport, and processing
   2. Direct smear preparation and microscopic examination
   3. Laboratory cultivation of bacteria
   4. Control of microorganisms
      1. Sterilization
      2. Disinfection
      3. Antisepsis
   5. Quality assurance in the clinical bacteriology laboratory
3. Categories and Classification of Bacteria
4. Ecology and Spread of Bacteria
5. Pathogenesis of Bacterial Infections
6. Catalase Positive Gram Positive Cocci
   1. Staphylococcus
   2. Micrococcus
7. Catalase Negative Gram Positive Cocci
   1. Streptococcus
   2. Streptococcus-like bacteria
8. Gram Negative Cocci
   1. Neisseria
   2. Moraxella
9. Enterobacteriaceae
   1. Clinical significant tribes and genera
   2. Proteeae
   3. Salmonella
   4. Shigella
   5. Citrobactereae
   6. Eschericheae
   7. Klebsiellae
   8. Yersiniae
10. Vibrionaceae
    1. Characteristics of the genus
    2. Vibrio cholerae
    3. Other vibrios
11. Campylobacter and Related Taxa
    1. Enteric campylobacters
    2. Other clinically significant campylobacters
    3. Arcobacter, helicobacter, and flexispira
12. Nonfermentative Gram Negative Bacilli
    1. Pseudomonads
    2. Acinetobacter
    3. Alcaligenes
13. Hemophilus
14. Miscellaneous Gram Negative Bacilli
    1. Gardnerella
    2. Legionella
    3. Bordetella
    4. Francisella
    5. Pasteurella
    6. Brucella
15. Aerobic Gram Positive, Non-Sporeforming Bacilli
    1. Corynebacterium
    2. Listeria
    3. Lactobacilli
16. Aerobic Spore-Forming Gram Positive Bacilli
    1. Bacillus anthrax
    2. Bacillus cereus
17. Clinically Significant Anaerobic Bacteria
    1. Collection and transport of specimens
    2. Procedures for isolation and characterization of anaerobes
    3. Clostridium genus
    4. Anaerobic gram negative bacilli
    5. Anaerobic gram positive cocci
18. Spirochetes
    1. Treponema
    2. Leptospira
    3. Borrelia
19. Methods in Antimicrobial Testing
    1. Principles and procedures
    2. Quality control
    3. Major classes of antibiotics and their mode of action
    4. Safety precautions
20. Mycobacteria
    1. Morphology and staining characteristics
    2. Pathogenic species
    3. Culture methods
21. Viruses and Other Microorganisms
    1. Viruses (e.g. cytomegalovirus, herpes simplex, influenza, varicella-zoster)
    2. Other microorganisms (e.g. chlamydia and mycoplasma)
22. Local Disease Processes
    1. Urinary tract
    2. Normally sterile body fluids
    3. Respiratory tract
    4. Genital tract
    5. Skin, soft tissues, joints, bone
    6. Intestinal disease
    7. Central nervous system
    8. Eye