ULAB 110B - Clinical Microbology Part 2 Laboratory

Perform the basic microbiology testing of concepts taught in 110A. Perform simulated cultures for urine, wound, and respiratory and/or throat samples. Demonstrate proper sterile technique in all work in the microbiology laboratory, prepare and interpret Gram stained slides, determine the acceptability of a culture specimen, and perform and document quality control in the clinical microbiology laboratory.

Prerequisite(s): ULAB 110A and Bachelor of Science with 30 credits of chemistry or biology.

Course Learning Outcomes:

Demonstrate and effectively implement sterile technique in all aspects of work in the clinical microbiology laboratory.
Set up cultures for evaluation using appropriate media and streaking techniques.
Properly prepare and evaluate Gram stained slides from both pure culture and clinical specimens.
Identify clinically significant microbial specimens from pure and mixed cultures using staining techniques, morphology, and biochemical reactions.
Accurately determine the presence of clinically significant organisms in a mixed culture.
Document and perform quality control in the clinical microbiology laboratory.

Outline:

Review the importance of proper sterile technique in the clinical microbiology lab.
- Practice streaking plates for isolated colonies.
- Prepare and Gram stained slides for evaluation.
- Set up plates for urine culture evaluation.
Evaluate Gram stained slides from pure cultures and clinical specimens.
- Evaluate streaked plates for proper isolation of colonies.
- Perform urine culture workup and identification.
- Set up plates for simulated polymicrobial/wound culture workup.
Isolate and subculture individual organisms from the simulated wound culture setup.
- Review molecular methodologies for organism identification and sensitivity testing.
- Review of antimicrobial agents and sensitivity testing.
Complete organism identification from wound culture simulation using:
- Gram stain reaction, morphology, and biochemical reactions.
- Set up plates for respiratory culture simulation.
Complete organism identification from respiratory culture setup.
- Perform throat swabs using rapid strep kits
- Perform and document QC for testing kits.
Perform lactophenol cotton blue stains of mold specimens
- Tease mounts and scotch tape mounts.
- Properly identify the unknown mold to genus level based on basic morphology.
Review parasitology slide preparations. Identify an unknown organism based on morphology.
Review of bacteriology techniques in preparation for final unknown.
- Set up plates, and make initial observations for culture and identification of final unknown organism.
- Complete identification of unknown organism using techniques covered in the laboratory course.