ULAB 105B - Immunohematology Part 2 Laboratory

Experience in performing ABO/Rh typing, antibody screens for detection of unexpected antibodies, antibody identification, antiglobulin testing using tube, gel and solid phase methods, blood product selection and preparation, compatibility testing, and quality control. Also includes routine testing and more complicated situations requiring additional testing to resolve unexpected results such as finding compatible units and adverse effects of blood transfusions. Quality assurance requirements for the blood bank will be reviewed. Students will perform qualify control (QC) on subject.

Prerequisite(s): ULAB 105A and Bachelor of Science with 30 credits of chemistry or biology

Course Learning Outcomes:

Perform ABO, Rh, and other blood group typing on blood samples.
Detect and identify unexpected antibodies using antiglobulin testing.
Prepare different blood products for transfusion including required pre-transfusion testing and post-transfusion workup of adverse events
Review qualify assurance and perform quality control for regulatory compliance in the blood bank.

Outline:

Review the principles of Immunology, complement, genetics and serological testing in Immunohematology.
Perform ABO and Rh typing.
Perform antibody (Ab) screens using the Indirect Antiglobulin Testing (IAT) using tube, gel and solid phase methods.
Perform Ab detection and Identification of Unexpected Antibodies
Become familiar with different blood products including Red Cells, Platelets, Fresh Frozen Plasma and Cryoprecipitate
Perform Pre-Transfusion Compatibility Testing of RBC units including phenotyping, as needed.
Perform an Adverse Effects of Blood Transfusion / Investigation work up using the Direct Antiglobulin Test (DAT)
Apply knowledge of Quality Assurance Procedures and Policies for review of products and performing QC
Perform a complete workup on an unknown sample: ABO/Rh, Ab screen, Ab ID, Ag typing, compatibility testing.